Abstract Detail

Molecular Ecology

Klein, Laura [1], Caito, Madeleine [2], Hopkins, Danielle [3], Kingeter, Christian [3], Knight, Erin [3], O'Hanlon, Regan [3], Pretz, Chelsea [4], Zander, Tracy [3], Miller, Allison [5].

Comparative digital morphometrics and genotyping-by-sequencing reveals species boundaries are maintained in central North American Vitis.

Interspecific hybridization is widely used in viticulture to produce more vigorous plants and is well documented in nature; however, the extent of gene flow among North American Vitis congeners within natural populations remains underexplored. The goal of this study was to investigate patterns of genetic structure and differentiation in two closely related species, V. riparia and V. rupestris, and their co-occurring congeners. Vitis riparia, the riverbank grape, and V. rupestris, the rock grape, have been particularly important in the development of rootstocks, with the majority of the cultivated grapevines now grafted to just a handful of V. riparia/V. rupestris cultivars. Sampling occurred at fourteen field sites in the Midwest United States. We also included a subset of previously identified germplasm accessions of any Vitis species that occur within the distributions of V. riparia and V. rupestris to confirm the identity of Vitis specimens collected at our field sites. We utilized genotyping-by-sequencing (GBS), yielding a dataset of 106,371 SNPs for 414 individuals. In our assessment of genetic structure, we detected eleven genetic clusters, consistent with the number of species included from the germplasm collection. Some genetic clusters were restricted to single collection sites, whereas other genetic clusters occurred in multiple sites. We observed evidence for some genetic admixture, but in general clusters represented distinct genetic groups in principal component analysis. This pattern of genetic differentiation in natural populations is coherent with existing Vitaceae phylogenetic relationships and morphological species descriptions for seven taxa. Leaf shape data analyzed using digital morphometric techniques largely support these species designations. These data support evidence for the role of species maintenance in the presence of interspecific gene flow and advocate for the examination of complex evolutionary histories through multiple means.

1 - Saint Louis University, Biology Department, 3507 Laclede Ave, Saint Louis, MO, 63103, United States
2 - Saint Louis University, Biology, 3507 Laclede Ave, Saint Louis, MO, 63103, USA
3 - Saint Louis University, 3507 Laclede Ave, Saint Louis, MO, 63103, United States
4 - University of Colorado-Boulder, 1900 Pleasant St, Boulder, CO, 80302, United States
5 - Saint Louis Univ./Danforth Plant Science Center, Biology, 3507 Laclede Avenue, Macelwane Hall, St. Louis, MO, 63110, United States

Keywords:
Genotyping-by-Sequencing
genomics
morphology
Vitis.

Presentation Type: Oral Paper
Session: MOL1, Molecular Ecology
Location: San Pedro 2/Starr Pass
Date: Wednesday, July 31st, 2019
Time: 4:00 PM
Number: MOL1010
Abstract ID:1026
Candidate for Awards:None