Abstract Detail

Reproductive Processes

Russell, Scott [1], Larson, Preston [2].

Use of cryoEM in assessing developmental mechanisms of rice reproduction.

Rice (Oryza sativa L.) is the most widespread crop in the world, and one that is useful for understanding reproductive mechanisms, as it is also a model plant for molecular biology and one for which -omic characteristics of flowering plant gametes are known. In addition to this significance, rice can also be used as a model for enhanced resolution electron microscopy that can be achieved using high resolution imaging of frozen material using cryotomographic equipment and sensitive direct electron imaging in resolutions that approach near atomic resolution under the best conditions. Such imaging may provide more sensitive imaging of dynamic developmental processes than are possible using traditional transmission electron microscopy. Such TEM images in the past are fraught with high damage, chemical artifacts of such fixatives as glutaraldehyde and osmium tetroxide and dehygration that provide significant artifacts. The current study represents an extension of these methods to achieve imaging that more closely records the native condition of tissues, cells and molecules at higher resolution.

1 - University Of Oklahoma, Department Of Microbiology And Plant Biology, 770 Van Vleet Oval, Norman, OK, 73019, United States
2 - University of Oklahoma, Samuel Roberts Noble Microscopy Laboratory, Norman, OK, 73019, USA

Keywords:
TEM
cryoEM
Oryza sativa
gamete structure
fertilization.

Presentation Type: Oral Paper
Session: REP2, Reproductive Processes 2
Location: San Luis 2/Starr Pass
Date: Wednesday, July 31st, 2019
Time: 2:30 PM
Number: REP2005
Abstract ID:1040
Candidate for Awards:None