Abstract Detail
Biodiversity Informatics & Herbarium Digitization Johnson, Bryson [1]. DNA Analysis of UVSC Herbarium Specimens and Comparison of CTAB and Kit Extractions by Bryson Johnson. The purpose of this project is to compare DNA extraction techniques on Utah Valley University Herbarium (UVSC) specimens. Destructive samples of the plant vouchers were used to extract DNA using both CTAB and Kit extraction methods. The extracted and purified DNA samples were assessed for quantity prior to PCR amplification using a nanodrop. The ITS region of the DNA samples were then amplified through PCR and correct amplification as confirmed through gel electrophoresis. Quantity was again assessed through nanodrop. Finally, the amplified PCR products were assessed for quality by sequencing the ITS region and analyzed using the software Geneious. The goal is to find a preferable method of DNA extractions on herbarium specimens and to add the sequenced data to DNA barcoding initiatives such as the International Barcode of Life. This will add potentially valuable knowledge that can be of benefit to scientist around the world. Log in to add this item to your schedule
1 - 1004 W Tuscany View Road D31, Midvale, UT, 84047, United States
Keywords: Barcoding herbarium Sequencing DNA Extraction.
Presentation Type: Poster Session: P, Biodiversity Informatics & Herbarium Digitization Posters Location: Arizona Ballroom/Starr Pass Date: Monday, July 29th, 2019 Time: 5:30 PM This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm. Number: PBI003 Abstract ID:307 Candidate for Awards:None |