Abstract Detail

Ethnobotany

Melo, Claudia [1], Cruz, Victor [1], Sorel, Victor [1], Cornejal, Nadjet [1], Buczynski-Kos, Aned C. [1], Priano, Christine [1], Fernández Romero, Jose [1], Koroch, Adolfina [1].

Biological Activity of Guava (Psidium guajava).

Guava (Psidium guajava) is a small tree native to South and Central America. Guava leaves, roots, and bark have been traditionally used for treating different illnesses. These benefits can be attributed to phenolics and flavonoids produced by guava for defense. We used different extraction methods for these chemicals and correlated its chemical composition with the biological activity. Total Phenolic, Total Flavonoids, ABTS/DPPH, TZM-bl, XTT, and 96 well microplate assays were used to test phenols, flavonoids, antioxidant properties, antiviral activity, cytotoxicity, and antibacterial activity, respectively. Genetically modified HeLa cells susceptible to two different HIV-1 strains were used in the antiviral procedure. The median cytotoxicity concentration (CC₅₀) and half-maximal effective concentration values (EC₅₀) were obtained through a curve-fitting analysis using GraphPad Prism Software, Inc. Therapeutic index (TI) was calculated using the CC₅₀/EC₅₀ ratio. Antibacterial activity against Escherichia coli was evaluated using a microplate assay. Results suggest that extraction of phenolic compounds is optimal when one gram of plant extract is dissolved in 10 mL of 60% ethanol/methanol followed by evaporation at 40° C and resuspension in 1 mL of 20% DMSO. The guava leaf extracts had a high phenol and flavonoid content that correlated with high antioxidant capacity and selective antiviral activity with TI values above 10. Preliminary results of antibacterial tests indicated that the extracts have bactericidal activity after 72 hours of exposure with bacteria. These results support the use of guava leaf extracts in traditional medicines.

1 - Borough of Manhattan Community College- CUNY, 199 Chambers Street, New York, NY, 10007, United States

Keywords:
guava
antioxidant capacity
antimicrobial activity
antiviral activity.

Presentation Type: Poster
Session: P, Ethnobotany Posters
Location: Arizona Ballroom/Starr Pass
Date: Monday, July 29th, 2019
Time: 5:30 PM This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PET005
Abstract ID:316
Candidate for Awards:Economic Botany Section best poster