Abstract Detail

Comparative Genomics/Transcriptomics

Lee, Chaehee [1], Ruhlman, Tracey [2], Jansen, Robert [3].

Multiple structural types of plastid genomes within a single individual.

It is widely accepted that plastid genomes (plastomes) of land plants have a conserved quadripartite structure consisting of two single copy regions separated by two copies of a large inverted repeat (IR), whereas mitochondrial genomes are highly variable in genome structure, having multiple genomic configurations including a master chromosome and sublimons due to active recombination mediated by repeats. Recently, alternative plastome structures have been suggested in Geraniaceae along with the co-existence of inversion isomers in some conifers and Medicago. We here report the complete plastome sequences of Eleocharisdulcis(Water chestnut) and E. cellulosa(Gulf coast spikerush) in the Cyperaceae, the second largest family of Poales. Unlike typical conserved plastomes of basal groups of Poales, Eleocharisplastomes have remarkably divergent features, including large genome sizes, higher rates of genome rearrangements, lower GC content, gene duplications, gene losses and increased repetitive DNA sequences. A novel finding among these features is the presence of multiple plastome structural types within a single individual. With the aid of the combination of Illumina paired-end and PacBio single-molecule real-time (SMRT) sequencing, Long Range PCR and Sanger sequencing data, at least four different plastome structural types are present in both species. PacBio long read data suggests that one of four plastome types of E. dulcis is likely to be the predominant type.

1 - The Universirt Of Texas At Austin, Department Of Integrative Biology, 1 University Station #C0930, BIO 225, Austin , TX, 78712, United States
2 - The Universirt Of Texas At Austin, Department Of Integrative Biology, 1 University Station #C0930, Austin, TX, 78712, USA
3 - University Of Texas At Austin, Integrative Biology, 205 W. 24th St. Stop C0930, Austin, TX, 78712, United States

Keywords:
plastome
multiple genomic configuration
Cyperaceae
repetitive DNA sequences
PacBio SMRT sequencing.

Presentation Type: Poster
Session: P, Comparative Genomics and Transcriptomics Posters
Location: /
Date: Monday, July 29th, 2019
Time: 5:30 PM This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PGT006
Abstract ID:361
Candidate for Awards:Genetics Section Poster Award