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Abstract Detail


Eserman, Lauren [1], Thomas, Shawn [2], Coffey, Emily [3], Leebens-Mack, Jim [4].

Target sequence capture in orchids: Developing a kit to sequence hundreds of single copy loci across all orchids.

Understanding relationships among orchid species and populations is of critical importance for orchid conservation. Target sequence capture has become a standard method for extracting hundreds of single copy orthologous loci for phylogenomics. However, the upfront cost and time associated with the design of a target capture kit makes this method prohibitively expensive for many researchers. Therefore, we have set out to design a target capture kit to reliably sequence hundreds of orthologous loci from across all orchids. We used a published set of orthologous loci from across orchids to identify genes that were single copy across the Orchidaceae. We then were left with 752 single copy genes including 5490 exons. We are testing the utility of this kit to reliably capture hundreds of single copy genes from species from most orchid subtribes. We hope that this kit will allow for greater accessibility of next generation sequencing for orchid systematics, population and conservation genetics, and, potentially, identification of poachers and other illegal orchid trade.

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1 - Atlanta Botanical Garden, Conservation & Research, 1345 Piedmont Ave NE, Atlanta, GA, 30309, United States
2 - University of Georgia, Plant Biology, 2502 Miller Plant Sciences, Athens, GA, 30602, United States
3 - Atlanta Botanical Garden, 1345 Piedmont Ave NE, Atlanta, GA, 30309, United States
4 - University Of Georgia, Plant Biology, 2101 Miller Plant Sciences, Athens, GA, 30602, United States

target enrichment
Next Generation Sequencing.

Presentation Type: Oral Paper
Session: PHYL1, Phylogenomics I
Location: San Ignacio/Starr Pass
Date: Monday, July 29th, 2019
Time: 8:45 AM
Number: PHYL1004
Abstract ID:391
Candidate for Awards:None

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