| Abstract Detail
Functional Genetics/Genomics Oram, Susa [1], Kenny, Cecile [1], Coate, Jeremy [1]. Genetic dissection of polyploidy-induced salinity tolerance in Arabidopsis thaliana. Polyploidy is correlated with enhanced salinity tolerance in a wide range of plant taxa but the molecular mechanisms for this phenotype remain unresolved. To address this question, we have generated isogenic diploid/autotetraploid pairs of Arabidopsis thaliana in several genetic backgrounds, including wildtype and various salt tolerance mutants (Salt Overly Sensitive [SOS] pathway genes, sos1, sos2 and sos3, and the High Affinity Potassium transporter, hkt1). Germination, growth and survival assays suggest that Arabidopsis polyploids achieve enhanced salt tolerance via a mechanism that is independent of the SOS pathway, but that appears to be dependent on HKT1. These observations are consistent with expression data showing that wildtype diploids upregulate the SOS pathway in response to stress whereas wildtype tetraploids do not, and that wildtype tetraploids constitutively overexpress HKT1 relative to their diploid parents. These data, as well as data from transcript-profiling and ionomic experiments, will be presented, and their implications for the mechanisms of polypoid-induced salt tolerance will be discussed. Log in to add this item to your schedule
1 - Reed College, 3203 SE Woodstock Blvd, Portland, OR, 97202.0, United States
Keywords: polyploidy Arabidopsis salt tolerance.
Presentation Type: Oral Paper Session: FG1, Functional Genetics and Genomics Location: San Luis 1/Starr Pass Date: Monday, July 29th, 2019 Time: 2:15 PM Number: FG1002 Abstract ID:813 Candidate for Awards:None |