| Abstract Detail
Pteridology Fauskee, Blake [1], Sigel, Erin [2], Grusz, Amanda [3]. A tale of three maidenhairs—variable frequencies of plastid RNA editing in Adiantum. Post-transcriptional modification of RNA, i.e., RNA editing, can lead to discordance between the nucleotide sequences observed in RNA transcripts and the nucleotide sequences present in the corresponding DNA template. In land plants, the modification of cytidines to uridines (C-to-U), known as forward RNA editing, is omnipresent; however, reverse RNA editing (U-to-C) appears to be restricted to ferns, lycophytes, and hornworts. Despite the propensity for forward and reverse RNA editing in land plant organellar genomes, editing sites are frequently inferred indirectly (e.g., by identifying premature stop codons). In this study, we directly compared DNA and RNA sequences from individual accessions of three congeneric taxa: Adiantum aleuticum, Adiantum shastense and Adiantum capillus-veneris (Pteridaceae). We characterized all plastid-derived RNA editing sites from A. aleuticum and A. shastense by employing a relaxed, automated variant-calling approach to minimize erroneous calls, combined with manual inspection to remove false positives. After characterizing plastid-derived RNA editing sites from A. aleuticum and A. shastense, we then compared these RNA editing sites to those previously confirmed in A. capillus-veneris. Our study is among the first to directly compare confirmed RNA editing frequency between three members of the same genus. Past studies have relied on PCR- or prediction-based methods to catalogue RNA editing sites; our study is unique in that it utilizes next generation sequencing of DNA and RNA sequences, in combination with a medley of bioinformatic tools. We find novel RNA editing sites, indicating that RNA editing is a highly variable process with the potential to influence expressed transcripts in relatively short evolutionary times scales.
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1 - 277 Lakehill Drive, Worthington, MN, 56187, United States
2 - University of Louisiana at Lafayette, Biology, 410 East St. Mary Blvd., Lafayette, LA, 70503, United States
3 - University Of Minnesota Duluth, Biology, 1035 Kirby Drive, SSB 207, Duluth, MN, 55812, United States
Keywords:
RNA editing
genomics
Transcriptomics
bioinformatics
pteridology.
Presentation Type: Oral Paper
Session: PTER1, Pteridology I
Location: San Pedro 1/Starr Pass
Date: Monday, July 29th, 2019
Time: 9:15 AM
Number: PTER1006
Abstract ID:886
Candidate for Awards:Edgar T. Wherry award |
