Abstract Detail

Comparative Genomics/Transcriptomics

Kehinde, Iyabode [1], Oyekanmi, Joshua [2].

Isolation and Characterization of some African Yam Bean (Sphenostylis sternocarpa Hochst ex. A. Rich Harms) fungal endophytes .

Endophytes are organisms that live between living plant cells establishing a relationship which varies from symbiotic to pathogenic. They have been found to be ubiquitously associated with almost all plants studied till date. They are often transmitted to the host plant vertically, that is from generation to generation through seeds or vegetative propagation. They produce compounds useful for plant growth, protection, prevents animals from grazing on the plants and are sometimes biocontrol agents on plants and of economic importance to human, Of all the world’s plants, probably only a few grass species endophytes have been well studied. Improvement of endophyte resources could bring about a variety of benefits, such as a novel, valuable and effective organic compounds that cannot be synthesized by chemical reactions. This may further assist in solving human, plant and animal health problems. Hence, the need to find new and interesting endophytes among the numerous plants in existence. Here, we give the isolation and identification of fungal endophytes associated with leaves, stems, and roots of African Yam Bean (AYB) (Sphenostylis stenocarpa) a leguminous plant classified as an underutilized crop but which can enhance food security of the poor thereby reducing hunger. This study investigated both cultural and molecular properties of endophytic fungi in AYB for its characterization and diversity. Fungal endophytes were isolated on PDA and pure cultures were culturally identified. DNA extraction, PCR amplification using ITS primers and analyses of nucleotide sequences of ribosomal DNA (rDNA) fragments were conducted on selected isolates most frequently encountered in farmers’ fields. Consensus nucleotide sequences of the fungi isolates were subjected to BLAST analysis and showed similar homology with genera of Rhizopus, Cunninghamella, Fusarium, Aspergillus, Penicillium, Alternaria, Diaporthe, Nigrospora, Purpureocillium, Corynespora, Magnaporthe, Macrophomina, Curvularia, Acrocalymma, Talaromyces, and Simplicillium. A slight similarity was found with endophytes associated with soybean. Many of these organisms have high economic importance in crop improvement and thus can be considered as endophytes that can offer some beneficial attributes to plant health, uptake of nutrients, disease suppression and host immunity.. Keywords: Fungal Endophytes, African Yam Bean, Internal Transcribed Spacer, Pest Control

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Endophytes

1 - Federal university of Agriculture, Abeokuta, Pure and Applied Botany, Alabata road, Abeokuta, Ogun, 1100011, Nigeria
2 - International Institute of Tropical Agriculture, Ibadan, Virology Unit, International Institute of Tropical Agriculture, Ibadan, Ogun, 1100011, Nigeria

Keywords:
fungal endophyte
African Yam Bean
Internal Transcribed Spacer.

Presentation Type: Poster
Session: P, Comparative Genomics and Transcriptomics Posters
Location: /
Date: Monday, July 29th, 2019
Time: 5:30 PM This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PGT017
Abstract ID:967
Candidate for Awards:None