| Abstract Detail
Comparative Genomics/Transcriptomics Field, Richard D [1], Heyduk, Karolina [2], Leebens-Mack, Jim [3]. In search of the regulatory basis of crassulacean acid metabolism (CAM): a comparative genomics approach in three species of Yucca L. Carbon concentration mechanisms (CCMs) are strategies used by plants to concentrate leaf carbon levels and increase photosynthetic and water-use efficiency (WUE). In crassulacean acid metabolism (CAM), carbon acquisition is confined to nighttime phases when temperatures, and thus transpiration, are lower than in the daytime. Though physiologically well characterized, a complete understanding of circadian CAM gene regulation is still lacking. Using three species from the genus Yucca L., we take a comparative genomics approach to uncover the evolutionary means by which plants repurpose conserved house-keeping genes into temporally-expressed carbon fixation genes. To this end, we combine information about chromatin accessibility, histone post-translational modifications, and gene expression, collected from 24-hour time course experiments, to construct CAM gene transcriptional networks. Our model system is ideal for comparative analyses because the three species differ in their use of CAM: Y. aloifolia does strong CAM, Y. filamentosa does not use a CCM, and Y. gloriosa, a hybrid of the other two species, switches to facultative weak CAM under drought stress. Previously phylogenetic analyses support Y. filamentosa as being representative of the ancestral type of carbon acquisition; thus, we will use our model to further elucidate both the regulatory requirements for CAM gene expression and the evolutionary trajectory by which plants tie CAM genes into the circadian clock. Log in to add this item to your schedule
1 - University of Georgia, Genetics, 120 W Green St, Athens, GA, 30602, USA 2 - Yale University, Department of Ecology and Evolutionary Biology, 165 Prospect Street, New Haven, CT, 06520, United States 3 - University Of Georgia, Plant Biology, 2101 Miller Plant Sciences, Athens, GA, 30602, United States
Keywords: genomics photosynthesis Gene Expression Chromatin Structure.
Presentation Type: Poster Session: P, Comparative Genomics and Transcriptomics Posters Location: / Date: Monday, July 29th, 2019 Time: 5:30 PM This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm. Number: PGT018 Abstract ID:980 Candidate for Awards:Genetics Section Poster Award |