Abstract Detail

Recent Topics Posters

David, Oyinade, A. [1], Osonubi, Oluwole [2], Oyetunji , Olusola [2].

Investigation of Physiological Mechanisms in Drought Tolerant Digitaria exilis L. (Fonio).

This study therefore investigated the mechanisms adopted by D. exilis to survive drought and identify the drought induced radicals and scavengers. Landraces Dinat Iburua (DID), Jakah Iburua (JAK) and Jiw Iburua (JIW), NG/11/JD/061 and NG/11/JD/062 were collected from recognized research institute. Murashige and Skoog medium of about 1.2 litres was supplemented with poly-ethylene glycol 6000 to create osmotic conditions of -9.29 MPa, -13.93 MPa, -20.13 MPa -26.32 MPa, -32.51 MPa and 0 MPa. After four weeks, plant growth parameters such as number of germinated seeds, leaves, tillers, leaf length, root and shoot weights were determined. Two kilograms of sterilized top soil was filled into polythene pots and thereafter, six landraces were planted. Plants were subjected to 0, 4, 8 and 12 days drought stress. Significant higher number of leaves, tillers, leaf length, dry root and shoot weights were recorded during the drought periods. Under osmotic stress, landrace JAK had the highest total chlorophyll contents (298.60 µg/ml), proline (0.052 mg/ml), APX (0.016 mmol/ml/min), SOD (1.659 units/mg protein), CAT (3.628 units/mg protein) compared with the low values of landraces DID and JIW. During drought stress, NG/11/JD/061 had the highest anthocyanin contents (1114.65µg/ml). Drought-stressed DID had higher dry weight (5.73 g) and DNA quantity (96 mg/µl) and lower lipid peroxidation (61%) than control plants. Landrace JAK had 33% drought tolerant ability than other landraces. Drought-stressed JAK scavenged 13% OH^- and 77% DPPH radicals than their controls. Leaf anatomical examination in landraces JAK and DID revealed the presence of turgid bulliform cells, intercellular spaces that ranged from 113µm -175 µm with compacted mesophyll cells while landrace NG/11/JD/062 had wider vessel (84.45 µm). Therefore, landrace JAK is highly drought tolerant and landraces DID and NG/11/JD/061 can be considered as mild drought tolerant whereas, landraces JIW and NG/11/JD/062 are highly susceptible to drought stress.

1 - Federal Uiversity Oye-Ekiti, Plant Science and Biotechnology, Oye, Ekiti, 371010, Nigeria
2 - University of Ibadan, Botany, Ibadan, Oyo, Nigeria

Keywords:
none specified

Presentation Type:
Session: P, Recent Topics Posters
Location: Arizona Ballroom/Starr Pass
Date: Monday, July 29th, 2019
Time: 5:30 PM
Number: PRT004
Abstract ID:1349
Candidate for Awards:None