| Abstract Detail
Recent Topics Posters Shen, Feng-Jiao [1], Shi, Shuo [1], Li, Lin [1], Wang, Dan [1], Wang, Meng-Zhen [1], Shevock, James R. [2], Zhao, Jian-Cheng [1]. Effects of Drying Methods on the DNA Quality of Bryophyte Specimens in the Field Collection. In the field, the preservation of bryophyte materials for high quality DNA extraction is a big challenge. Traditionally it is natural-dry and lately silica gel-dry. Recently we used hot air drying methods on trial. In this study, the DNA qualities extracted from bryophyte specimen using several drying methods, natural-dry, hot air-dry (40℃, 80℃ and 150℃) and silica gel-dry, were compared. The quantity of extracted genomic DNA, ratios of D260 to D230, the success rate of PCR, suggested that the hot air-dry (40–80℃) was the best. The hot air-dry methods have no serious negative effects on plant morphology and the specimens are usable for taxonomic studies. Hot air-drying treatments are convenient to be done where there is electricity. Several devices such as portable hair dryer, fan heater, or electric blanket are cheaply available. Such methods avoid rapid DNA degradation and silica gel pollution, and save the manpower for carrying a large number of silica gels in the field.
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1 - Hebei Normal University, College of Life Sciences, No.20, East Nanerhuan Road, Shijiazhuang, Hebei, 050024, China
2 - California Academy of Sciences, Department of Botany, 55 Music Concourse Drive, Golden Gate Park, San Francisco, CA, 94118, USA
Keywords:
bryophyte
Field Work
molecular specimen
DNA specimen
hot-air drying
silica gel
natural drying
animal.
Presentation Type:
Session: P, Recent Topics Posters
Location: Arizona Ballroom/Starr Pass
Date: Monday, July 29th, 2019
Time: 5:30 PM
Number: PRT021
Abstract ID:1398
Candidate for Awards:None |
