Abstract Detail

Population Genetics/Genomics

Diaz, Nicolas [1], Thompson, Pamela [2], Cruzan, Mitchell [3].

Characterizing the genome size variation, morphology, and chloroplast haplotype diversity in mixed ploidy populations of Eriophyllum lanatum.

Eriophyllum lanatum, or Oregon sunshine, is a species complex with a range extending from southern California to British Columbia, and as far east as Montana and Wyoming. This phenotypically diverse group is comprised of geographically delineated varieties that intergrade at the interfaces of their ranges. Previous cytogeographic work by J.S. Mooring in 2008 revealed a contact zone between E. lanatum var. leucophyllum and var. achilleoides in Southern Oregon that consists of morphologically intermediate diploid, tetraploid, and hexaploid populations. In 2018 we used flow cytometry on 5 individuals across 22 populations of E. lanatum in Southern Oregon to validate the persistence of this polyploid contact zone. We paired this analysis of ploidy variation with an analysis of chloroplast haplotype diversity among these populations and 5 new populations. Following a de novo chloroplast genome assembly, we used the recently developed CallHap pipeline to reconstruct haplotype frequencies from SNP data, and discovered 47 haplotypes. In a subsequent field excursion, we revisited the study site and used direct observation and digital measurements in ImageJ to measure peduncle length, number of flowers, leaf arrangement, ray petal length, ray petal width, flower head diameter, and size of inner target color. We discovered populations made up of multiple cytotypes with considerable variation in morphology that is not explained by genome size. In 2019 we returned to the mixed ploidy populations to determine the relative proportions of these cytotypes using flow cytometry and to screen for rare variants.

1 - Portland State University, Biology Department, 1719 SW 10th Avenue, SRTC Rm 246, Portland , OR, 97201, United States
2 - Portland State University, Biology, 1719 SW 10th Ave., SRTC Rm. 246, Portland, OR, 97201, USA
3 - Portland State University, Department Of Biology, 1719 SW 10th Ave, SRTC Rm 246 - Biology, Portland, OR, 97201, United States

Keywords:
polyploidy
Mixed-ploidy populations
CallHap
Plastid population genomics.

Presentation Type: Poster
Session: P, Population Genetics/Genomics Posters
Location: Arizona Ballroom/Starr Pass
Date: Monday, July 29th, 2019
Time: 5:30 PM This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PPG010
Abstract ID:600
Candidate for Awards:None