| Abstract Detail
Comparative Genomics/Transcriptomics Garza, Elyssa [1], Hawkins, Angela [2], Pepper, Alan [3]. Using comparative genomics to uncover the secrets of the serpentine jewelflower: Caulanthus amplexicaulis. Serpentine soils are characterized by limiting nutrients (e.g. Ca, N, P, K), low water retention, and high levels of toxic heavy metals. The diploid jewelflower, Caulanthus amplexicaulis var. barbarae (Cab), is endemic to these soils and has developed strategies to overcome these stressful conditions and sustain a small population in serpentine-containing areas. To understand the changes accrued in the stressed Caulanthus genome over time, the Cab genome was compared to its granite-living sister variety, Caulanthus amplexicaulis var. amplexicaulis (Caa). We have assembled, analyzed, and annotated both Cab and Caa genomes (~372 Mb size; 14 chromosomes). Genomic datasets from Recombinant Inbred Lines (RILs) and parental plants will be combined using several bioinformatic tools to construct a high-resolution linkage map of the Caulanthus genome. This map will serve as a reference to incorporate RNA-Seq expression data and QTL phenotypic data, which will allow the identification of candidate genes involved in serpentine endemism and tolerance.
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1 - Texas A&M University, Department of Biology, 400 Bizzell St,, College Station, TX, 77843, United States
2 - Texas A&M University, Department Of Biology, 3258 TAMU, College Station, TX, 77843, United States
3 - Texas A&M University, Department Of Biology, Texas A&M University, TAMU 3258, 214 Bsbe, College Station, TX, 77843, United States
Keywords:
genome
serpentine
Caulanthus
Sequencing.
Presentation Type: Oral Paper
Session: CG1, Comparative Genetics/Genomics I
Location: Tucson I/Starr Pass
Date: Tuesday, July 30th, 2019
Time: 10:15 AM
Number: CG1007
Abstract ID:811
Candidate for Awards:Margaret Menzel Award |
