Abstract Detail

Crops and Wild Relatives

Onuminya, Temitope [1], Ogundipe, Oluwatoyin [1], Lawal, Oyewole [1], Ogunkanmi, Mubarak [1].

Morphological and Molecular Characterization of Cowpea Accessions from Africa.

Morphological and Molecular characterization are crucial tools in assessing the level of genetic diveristy and variation in plant species. In this study, genotypes of cowpea (Vigna unguiculata (L) Walp.) from ten African countries were collected from International Institute for Tropical Agriculture (IITA). The genotypes were planted in a randomized complete block design with four replicates. Morphological characters of seedlings were assessed following the International Board for Plant and Genetic Resource Cowpea Descriptor list and both quantitative and qualitative characters were evaluated. Results showed that the plant habit was primarily sem-erect with intermediate twining (80%) while others have erect and slightly twining habit (20%). Pigmentation ranges from extreme (n=1), moderate= (n=1), slight = (n=16) to none (n=2). Flowers color is either pink (65%), white (20%) or violet (15%). Inflorescence is raceme. Pod is straight (20%) or curved (80%). Peduncle length ranges from 1.87 to 15.5 cm, pod length is 8.63-18.83 cm while seed is 6.10-9.93mm by 3.35-5.75 mm. Analysis of the morphological data using UPGMA showed a dendogram with four (4) clusters. Genomic DNA was successfully extracted from the seedlings with relative absorbance of 1.87 and concentration of 339-923 ng/ul. All the samples were amplified using six SSR markers (SSR27BY, SSR19BY, SSR10BY, SSR9BY, SSR8BY and SSR21BY) however only three successfully amplified. A total of 7 alleles were observed while the PIC ranges from 0.868 to 0.992. Assessment of relationship based on molecular characters revealed a dendogram with two clusters and one outgroup. This study revealed low genetic diversity among the accessions and a relatively high variation based on morphological characters. Agro-morphological traits (quantitative and qualitative) evaluated in this studies proved valuable for cowpea genetic diversity studies. This project is sponsored by the Tertiary Education Trust Fund (TETFUND).

1 - University of Lagos, Department of Botany, Akoka, Yaba, Lagos, 23401, Nigeria

Keywords:
Cowpea
genetic diversity
Dendogram
Africa.

Presentation Type: Poster
Session: P, Crops and Wild Relatives Posters
Location: Arizona Ballroom/Starr Pass
Date: Monday, July 29th, 2019
Time: 5:30 PM This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PCW001
Abstract ID:86
Candidate for Awards:None