Abstract Detail

Crops and Wild Relatives

Gallagher, Evan [1], Yuxiang, Yuan [2], Mabry, Makenzie [3], Pires, Joseph [4].

Breeding the Dog of the Plant World: Development of Doubled Haploid Brassica oleracea.

Just like dogs are known for their tremendous diversity in size and shape due to domestication­, Brassica oleracea is one plant species that has been domesticated into many familiar crops. These include vegetables such as Broccoli, Brussels sprouts, Cabbage, Cauliflower, Kale, and Kohlrabi. The challenge with improving these vegetables is that there are not publicly available inbred lines that can be used to develop elite germplasm, as has been done in corn (maize) and other crops. One way to make inbred lines of diploid organisms is to instantaneously double the genome of male or female gametes. We have developed and optimized the process of making doubled haploids (DH) lines to reduce the time spent selfing plants into homozygosity from many generations to one. At present, there are only a few DH lines publicly available for each of these important crops, with most of them being kales. Improving upon previous techniques and producing doubled haploids for each of these six major morphotypes allows for studies to assess the genotype to phenotype relationship on completely homozygous lines within a single species.

1 - University of Missouri-Columbia, Life Science Center 311, 1201 Rollins St, Columbia, MO, 65201, USA
2 - University of Missouri-Columbia, Life Science Center 311, 1201 Rollins St, Columbia, MO, 65201, United States
3 - University Of Missouri, Biological Sciences, 1201 Rollins St., Columbia, MO, 65201, United States
4 - University Of Missouri, 371 Bond Life Sciences Center, 1201 Rollins Street, Columbia, MO, 65211, United States

Keywords:
Doubled Haploid
Microspore Culturing
Brassica oleracea
homozygous.

Presentation Type: Poster
Session: P, Crops and Wild Relatives Posters
Location: Arizona Ballroom/Starr Pass
Date: Monday, July 29th, 2019
Time: 5:30 PM This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PCW004
Abstract ID:284
Candidate for Awards:None